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Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate

Details
Host / Isotype Goat / IgG Class Polyclonal Type Secondary Antibody Tested species reactivity Mouse Immunogen Gamma Immunoglobins Heavy and Light chains Conjugate HRP Target Class IgG (H+L) Cross Adsorption Against bovine, horse, human, pig and rabbit serum proteins Antibody Form Whole Antibody
Tested Applications Dilution * ELISA (ELISA) 1:500-1:20,000 Immunohistochemistry (IHC) 1:500-1:20,000 Western Blot (WB) 1:500-1:20,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product specific information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Form information
Form Lyophilized Purification Affinity chromatography Storage buffer PBS, pH 7.2, with 1% BSA Preservative 0.1% Kathon™ CG Storage Conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Gene/Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.