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  • Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate

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Details

Host / IsotypeGoat / IgG
ClassPolyclonal
TypeSecondary Antibody
Tested species reactivityMouse
ImmunogenGamma Immunoglobins Heavy and Light chains
ConjugateHRP
Target ClassIgG (H+L)
Cross AdsorptionAgainst bovine, horse, human, pig and rabbit serum proteins
Antibody FormWhole Antibody
Tested ApplicationsDilution *                                   
ELISA (ELISA)1:500-1:20,000
Immunohistochemistry (IHC)1:500-1:20,000
Western Blot (WB)1:500-1:20,000


* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.


Product specific information

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


Form information

FormLyophilized
PurificationAffinity chromatography
Storage bufferPBS, pH 7.2, with 1% BSA
Preservative0.1% Kathon™ CG
Storage ConditionsStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.


Gene/Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.


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