SYBR® Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

描述

SYBR® Gold nucleic acid gel stain is the most sensitive fluorescent stain offered by Life Technologies for the detection of nucleic acids. Use with UV transilluminators, the Dark Reader (see stained nucleic acid gel below), laser scanners, or blue light transilluminators such as the Safe Imager™ 2.0 or the E-Gel® Imager.

• Ultra Sensitive: 25 – 100 times more sensitive than ethidium bromide. Detect as little as 25 pg of DNA
• Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage
• Increased Signal to Background Ratio: More than 1000-fold signal enhancement when bound to nucleic acids
• Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels

Stain Properties
SYBR® Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (~0.6) upon binding to double- or single-stranded DNA or to RNA1. Excitation maxima for dye-nucleic acid complexes are at ~495 nm and ~300 nm and the emission maximum is ~537 nm (see spectra).

Stain Sensitivity
SYBR® Gold stain is >10-fold more sensitive than ethidium bromide for detecting DNA and RNA in denaturing urea, glyoxal, and formaldehyde gels, even with 300 nm transillumination (see visualization of glyoxalatd RNA below). SYBR® Gold stain has also been shown to be much more sensitive than SYBR® Green II stain for detecting single strand conformation polymorphism (SSCP) products (see figure below). Furthermore, it is much more sensitive than silver-staining for the detection of double-stranded DNA in native polyacrylamide gels (see sensitivity comparison below).

1Tuma RS, Beaudet MP, Jin X, Jones LJ, Cheung CY, Yue S, Singer VL. Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem (1999) 268:278-288. (PMID: 10075818)

For research use only. Not for human or animal therapeutic or diagnostic use.

规格

Target Molecule:	DNA, RNA
Label or Dye:	SYBR® Gold
Detection Method:	Fluorescent
Detection Location:	In-Gel Detection
Product Size:	500 µL
Shipping Condition:	Room Temperature

内容及储存

Supplied as a 10,000X concentrate in DMSO. Store at -20°C, protected from light in a dessicator.

Comparison of glyoxalated RNA stained with ethidium bromide and with the SYBR® Gold Nucleic Acid Gel Stain.
Comparison of glyoxalated RNA stained with ethidium bromide and with the SYBR® Gold Nucleic Acid Gel Stain (Cat. No. S11494). Identical two-fold dilutions of glyoxalated Escherichia coli 16S and 23S ribosomal RNA were separated on 1% agarose minigels using standard methods and stained for 30 min with SYBR® Gold stain in TBE buffer (panel A) or with 0.5 µg/mL ethidium bromide in 0.1 M ammonium acetate (panel B). Both gels were subjected to 300 nm transillumination and photographed using Polaroid 667 black-and-white print film and a SYBR® photographic filter (Cat. No. S7569, panel A), or an ethidium bromide photographic filter (panel B).

Sensitivity of SYBR® Gold stain compared to silver stain.
Comparison of the sensitivity achieved using SYBR® Gold stain (Cat. No. S11494) with 300 nm transillumination (panel A) and silver stain to detect double-stranded DNA separated on native polyacrylamide gels (panel B).

Single-strand conformation polymorphism (SSCP) in exon 1 of human K-ras.
Sensitive and direct visualization of single-strand conformation polymorphism (SSCP) in exon 1 of human K-ras using SYBR® Gold Nucleic Acid Gel Stain (Cat. No. S11494). Lane 1 contains wild-type DNA and lanes 2–4 contain DNA from various adenocarcinoma samples with mutant alleles. Image contributed by Valerie DeGroff and Chris Weghorst, Ohio State University.

SYBR® Gold Nucleic Acid Gel Stain.
A two-fold dilution series of HindIII-digested lambda DNA was separated by electrophoresis through an agarose gel and stained with SYBR® Gold Nucleic Acid Gel Stain (Cat. No. S11494). Gel staining was visualized using the Dark Reader transilluminator (Clare Chemical Research).

荧光光谱

SYBR Gold
Fluorescence Ex/Em spectra of SYBR® Gold nucleic acid gel stain bound to DNA.

   Open in SpectraViewer

手册和实验方案  

• SYBR Gold Nucleic Acid Gel Stain

• SYBR Nucleic Acid Gel Stains — Useful Tips

Molecular probes手册 

• Nucleic Acid Stains—Section 8.1 

• Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4

• 
  

• 
  

化学品安全技术说明书(MSDS)

• SYBR® Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

• 
  

分析证书（COA）

• 批号 1743260

• 批号 1730968

• 批号 1702498

• 批号 1683656

• 批号 1616936

• 批号 1351929

• 批号 29704W

• 批号 43928A

• 批号 927072

• 批号 824968

• 批号 1246112

• 批号 1704606

• 批号 895273

• 批号 1656115

• 批号 1507890

• 批号 1575893

• 批号 18E1-3

• 批号 1099926

• 批号 612365

• 批号 1126452

• 批号 950657

• 批号 1007808

• 批号 1308457

• 批号 1170128

• 批号 439642