Proteinase K Powder (≥40 U/mgP)

描述

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.

规格

Form:	Powder
Product Size:	100 mg
Shipping Condition:	Approved for shipment at Room Temperature or on Wet Ice

内容及储存

One vial

Store in refrigerator (2–8°C).

手册和实验方案

• Proteinase K

• 
  

化学品安全技术说明书(MSDS)

• Proteinase K Powder (≥40 U/mgP)

分析证书（COA）

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