Phusion Site-Directed Mutagenesis Kit

产品概述

描述

Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.


Highlights

• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
• Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competent E. coli cells

规格

Mutagenesis Capability:	Single-Site Mutagenesis
Product Size:	20 reactions

Figures

手册

手册和实验方案

• User Guide: Phusion Site-Directed Mutagenesis Kit

• 
  

化学品安全技术说明书(MSDS)

• Phusion Site-Directed Mutagenesis Kit

• 
  

引用及参考文献

 Sequence and structure evolved separately in a ribosomal ubiquitin variant

A. Catic et al.
EMBO J (2007) 26(14):3474-3483