Mutation Generation System Kit

产品概述

描述

Thermo Scientific transposon products are based on the transposition machinery of the bacteriophage Mu. During the lytic phase of the phage's life cycle the machinery replicates its genome by transposing repeatedly inside the host genome. The Mu transposition reaction has been modified into an in vitro reaction catalyzed by a single enzyme - MuA Transposase. In this system, one in vitro reaction is capable of generating more than a million transposon insertion clones.

The Mutation Generation System (MGS Kit) and Stop Generation System (STOP Kit) were developed for functional analysis of proteins. These new transposon tools enable the creation of saturated libraries of mutated proteins in a single reaction with less hands-on time than any other method. The location of the transposon insertion in each mutated clone can be mapped by either PCR or sequencing. With MGS and STOP kits, thousands of mutated clones are ready for expression studies in just 2 to 3 days.

规格

Mutagenesis Capability:	Mutagenesis at Multiple Sites
Product Size:	10 reactions

Figures

手册

手册和实验方案

• User Guide: Mutation Generation System Kit

• User Guide: Stop Generation System

化学品安全技术说明书(MSDS)

• Mutation Generation System Kit

引用及参考文献

Expression of green fluorescent protein in Bacillus brevis under the control of a novel constitutive promoter of F1 in Escherichia coli DH5a

Y. Chen et al.
FEMS Microbiol Lett (2003) 229(1):111-117

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Probing the alpha-Complementing Domain of E. coli beta-Galactosidase With Use of an Insertional Pentapeptide Mutagenesis Strategy Based on Mu in vitro DNA Transposition. 

E. Poussu et al.
Proteins (2004) 54(4):681-692

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Analysis of Human Pex19p’s Domain Structure by Pentapeptide Scanning Mutagenesis

M. Fransen et al.
J Mol Biol (2005) 346(5):1275-1286