MessageSensor™ RT Kit

产品概述

描述

For the reverse transcription of RNA. The Ambion® kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions. 
• Linear quantitation of mRNA targets over 106-fold range of input RNA 
• Quantitate transcripts from as little as 500 fg total RNA—1⁄20th of a single cell 
• Fast, flexible RT step complete in 15 min; use reaction temperatures up to 50°C 
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods 
MessageSensor™ is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity (see figure). The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than 2 hours.

规格

Reverse Transcriptase:	M-MLV Reverse Transcriptase
Downstream Application:	Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	50 reactions
Final Product:	cDNA
Shipping Condition:	Dry Ice
Final Product Size(s):	7 kb or less
Sample Type (Specific):	mRNA
Optimal Reaction Temperature:	42-50° C

内容及储存

10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, and hGAPDH Control Primers should be stored at –20°C. HeLa S3 Control RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.

Figures

手册

手册和实验方案

• MessageSensor™ RT Kit Instruction Manual

化学品安全技术说明书(MSDS)

• MessageSensor™ RT Kit