Gateway® Vector Conversion System with One Shot® ccdB Survival Cells

产品概述

描述

The Gateway® Vector Conversion System is designed to convert any vector into a Gateway® destination vector using restriction endonucleases and ligase. A Gateway® cassette containingattR recombination sites flanking a ccdB gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccdB gene. The Gateway® Vector Conversion System offers: 
• Conversion of any expression vector into a Gateway® destination vector
• Three cassettes to construct a destination vector in the correct reading frame. Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation.

规格

Product Size:	1 kit
Cloning Method:	Gateway®
Bacterial or Yeast Strain:	ccdB Survival™ 2
Antibiotic Resistance (Bacterial):	Chloramphenicol (CmR)

内容及储存

The Gateway® Vector Conversion System contains Gateway® Reading Frame Cassettes A, B, and C.1, pENTR™-gus positive control, and One Shot® ccdB Survival Cells. Store DNA at -20°C and competent cells at -80°C.

Figures

手册

手册和实验方案

• User guide: Gateway Technology pgs 1-9 (Japanese)

• Gateway® Vector Conversion System with One Shot® ccdB Survival™ 2 T1R Competent Cells

• User Guide: Gateway Technology

化学品安全技术说明书(MSDS)

• 100002248

• 460700

• 52918

• 52919

• 52920

• 53533

• 54357

• 
  

引用及参考文献

High-throughput gateway bicistronic retroviral vectors for stable expression in mammalian cells: exploring the biologic effects of STAT5 overexpression.

Royer Y, Menu C, Liu X, Constantinescu SN, 
DNA Cell Biol (2004) 23:355-365
Product usage: Customer converted several bicistronic retroviral vectors containing a variety of different genes downstream of an IRES (GFP, CD2, or CD4) as reporters, to Gateway destination vectors in order to facilitate high-throughput cloning of any number of genes. They transferred EpoR via

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Trpm7 regulates cell adhesion by controlling the calcium dependent protease calpain.

Su LT, Agapito MA, Li M, T N Simonson W, Huttenlocher A, Habas R, Yue L, Runnels LW, 
J Biol Chem 2006 0(0):-
Product usage: The Flp-in T-Rex system was used in conjunction with Flp-in T-Rex 293 cells for regulated, isogenic expression of the ion channel-kinase, TRPM7 and TRPM7 kinase mutants as well as shRNAs targeted to TRPM7. This helped in testing the function of the kinase domain and in determinin

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A modified Gateway cloning strategy for overexpressing tagged proteins in plants.

Dubin MJ, Bowler C, Benvenuto G, 
Plant Methods (2008) 4:3-3
Product usage: An alternative Gateway-based strategy is reported that has been developed to create N- or C-terminally tagged protein overexpression constructs that lack attB-derived linker amino acids between the protein of interest and the tag. The attB sequences are avoided because the tag is