TaqMan® MicroRNA Reverse Transcription Kit, 1000 Reactions

产品概述

描述

Applied Biosystems® TaqMan® MicroRNA反转录试剂盒提供最佳性能的基于TaqMan® MicroRNAAssay反应所需的所有成分。该试剂盒包含200个反应，试剂盒中的成分可结合TaqMan® MicroRNA Assay附带的反转录引物，从而将miRNA转换为cDNA。

这是一种高度特异性的试剂盒，可保证仅对成熟miRNA进行逆转录，不对miRNA前体反应。作为一种敏感的试剂盒，其可对有限的样品进行反应，仅需1-10 ng的总RNA。适用于快速、简单且可扩展的2步法定量RT-PCR定量分析，从而在不到三小时内提供优质的分析结果。

规格

Reverse Transcriptase:	MultiScribe™ MuLV
Downstream Application:	Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	1,000 reactions
Final Product:	cDNA
Shipping Condition:	Dry Ice
Final Product Size(s):	200 bp or less
Optimal Reaction Temperature:	42° C

内容及储存

Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/µL), and MultiScribe™ RT enzyme (50U/µL) for 1000 reactions. Store at -15 to -25°C.

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手册

手册和实验方案

• Protocol: TaqMan® Small RNA Assays Protocol (English )

• Prod Insert: Megaplex Primer Pools - Megaplex RT Primers, Megaplex PreAmp Primers

产品文献

• Brochures & Specifications: TaqMan® MicroRNA Assays and Arrays: Convenient, scalable solutions for miRNA quantitation and profiling (English )

• Application Notes & Tutorials: High correlation of miRNA quantitation data from matched FFPE and snap-frozen tissues using TaqMan MicroRNA Assays

科研海报

• Poster: microRNA Quantitation by Real-time PCR

• Poster: miRNA Expression Profiling in Brain Tumor by Real-Time PCR

• Poster: Plant microRNA Quantitation by Real-time PCR

• Poster: TaqMan-based miRNA Profiles Classify Embryonic Stem and Differentiated Cells

化学品安全技术说明书(MSDS)

• 4319981

• 4319983

• 4367381

• 4469082

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