TaqMan® Reverse Transcription Reagents

产品概述

描述

TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:

• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with one-step or two-step RT-PCR reactions

Using TaqMan® Reverse Transcription Reagents
TaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Built-in normalization
The TaqMan® reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

规格

Reverse Transcriptase:	MultiScribe™ MuLV
Downstream Application:	Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	2,000 reactions
Final Product:	cDNA (First-Strand)
Final Product Size(s):	5 kb or less
Optimal Reaction Temperature:	42° C

内容及储存

Contents: MultiScribe™ Reverse Transcriptase (100 µL at 50U/µL). Store for up to one year at -20°C.

A note about the size: This kit contains enough reagents for 2000 reverse transcriptase reactions when the reactions are set up at 10 µL each.

手册

手册和实验方案

• User Guide: TaqMan Reverse Transcription Reagents

产品文献

• Essentials of Real Time PCR

• Application Tutorial: Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines

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化学品安全技术说明书(MSDS)

• N8080234COMPONENTA

• N8080234COMPONENTB

• N8080234COMPONENTC

• N8080234COMPONENTD

• N8080234COMPONENTE

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引用及参考文献

Combined analysis of levels of serum B-cell activating factor and a proliferation-inducing ligand as predictor of disease progression in patients with chronic lymphocytic leukemia.

Ferrer G, Hodgson K, Pereira A, Juan M, Elena M, Colomer D, Roué G, Aymerich M, Baumann T, Montserrat E, Moreno C
Leuk Lymphoma 2011; (52):11 2064-2068

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Regulating type 1 IFN effects in CD8 T cells during viral infections: changing STAT4 and STAT1 expression for function.

Gil MP, Ploquin MJ, Watford WT, Lee SH, Kim K, Wang X, Kanno Y, O'Shea JJ, Biron CA
Blood 2012; (120):18 3718-3728