Verso cDNA Synthesis Kit

产品概述

描述

Thermo Scientific Verso cDNA synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Highlights

• Highly sensitive with a broad dynamic range: can reverse transcribe RNA from an input range of 1pg to 1µg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42°C to 57°C) for success with GC-rich and other difficult templates
• Included RT Enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase Inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR

Applications

• 2-step RT-(q)PCR
• Single stranded cDNA synthesis

Related Products
Verso cDNA Synthesis Kit

规格

Reverse Transcriptase:	Verso Reverse Transcriptase
Downstream Application:	Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	40 reactions
Optimal Reaction Temperature:	42° C

内容及储存

Verso cDNA Synthesis Kit includes RT Enzyme mix with RNase Inhibitor, 5X RT Buffer, Random Hexamers, Anchored Oligo dT, 20 mM dNTPs, and RT Enhancer.

Store at -20°C.

手册

手册和实验方案

• User Guide: Verso cDNA Synthesis Kit, AB1453A

化学品安全技术说明书(MSDS)

• Verso cDNA Synthesis Kit