DyNAmo cDNA Synthesis Kit

产品概述

描述

Thermo Scientific DyNAmo cDNA Synthesis Kit is intended for cDNA synthesis in two-step reverse transcription-quantitative PCR (RT-qPCR) applications, where amplicons are usually around 100 bp in length. This kit can be used in conjunction with any qPCR master mix.

The DyNAmo cDNA Synthesis Kit includes a M-MuLV RNase H+ reverse transcriptase and all other necessary reagents for cDNA synthesis prior to qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers as separate vials, offering users flexibility in their choice of priming strategy, including the option to use gene-specific primers.

Highlights

• Broad dynamic range—up to 1 µg of RNA
• Reliable results with different RNA sources—mRNA, total RNA, viral RNA, and in vitro transcribed RNA
• Flexible—all priming options for cDNA synthesis
• Convenient to use—Short protocol
• Optimized 2X mix for cDNA synthesis

Applications

• RT-qPCR

规格

Reverse Transcriptase:	M-MuLV RNase H+ Reverse Transcriptase
Downstream Application:	Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	100 reactions
Optimal Reaction Temperature:	37° C

内容及储存

Contains:

• M-MuLV RNase H+ reverse transcriptase (includes RNase Inhibitor)
• 2X RT buffer (includes dNTP mix and 10 mM MgCl2)
• Random hexamers (300 ng/µL)
• Oligo(dT)15 primer (100 ng/µl)

Store at -20°C.

手册

手册和实验方案

• User Guide: DyNAmo cDNA Synthesis Kit

化学品安全技术说明书(MSDS)

• VNF470L

• VNF470X

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引用及参考文献

Telomere dysfunction in human keratinocytes elicits senescence and a novel transcription profile

F. Minty et al.
Exp Cell Res (2008) 314(13):2434-2447