RevertAid H Minus First Strand cDNA Synthesis Kit

产品概述

描述

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Increased reaction temperatures in the range of 42 to 55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit—oligo(dT)18 and random hexamer primers included with the kit

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis 

Additional Features
The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, realtime PCR or in second strand cDNA synthesis.

规格

Reverse Transcriptase:	RevertAid H Minus Reverse Transcriptase
Downstream Application:	Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	20 reactions
Optimal Reaction Temperature:	42-45° C

内容及储存

The RevertAid H Minus First Strand cDNA Synthesis Kit contains:
• RevertAid H Minus Reverse Transcriptase,
• RiboLock RNase Inhibitor,
• 5X Reaction Buffer,
• dNTP Mix,
• Oligo(dT)18 Primer,
• Random Hexamer Primer,
• Control RNA,
• Control Primers and 
• Nuclease-free water.

Store at -20°C.

手册

手册和实验方案

• User Guide: Guidelines to Avoid RNase Contamination

• User Guide: RevertAid H Minus First Strand cDNA Synthesis Kit, 20 reactions

• User Guide: Removal of Genomic DNA from RNA Preparations

化学品安全技术说明书(MSDS)

• VNK1631

• VNK163X

• VNL095

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引用及参考文献

UPS1 and UPS2 from Arabidopsis Mediate High Affinity Transport of Uracil and 5-Fluorouracil

A. Schmidt et al.
J Biol Chem (2004) 279:44817-44824

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Deletion of the Mycobacterium tuberculosis pknH Gene Confers a Higher Bacillary Load during the Chronic Phase of Infection in BALB/c Mice

K. G. Papavinasasundaram et al.
J Bacteriol (2005) 187(16):5751-5760