3' RACE System for Rapid Amplification of cDNA Ends

产品概述

描述

3´ RACE 系统 (3´ RACE System) 适合用于 cDNA 末端的快速扩增 (RACE) (1-3) 和 mRNA 中确定的点与 3´ poly(A) 末端之间的锚式 PCR（图 1）。 系统可用于扩增序列信息较少的罕见 mRNA，还可用于捕获 mRNA 的 3´ 末端信息。 3´ RACE 系统：

• 能和 5´ RACE 系统一起使用，克隆全长序列

• 利用 SuperScript® II RT 的先进性能，获得更高的第一链 cDNA 产量

• 包括对照 RNA 和引物，监控系统性能

• 在说明书中提供详细方案

• 在一个反应中可将多达 1 µg 的总 RNA 或 100 ng 对照 RNA 转化成 cDNA

规格

Product Size:	20 reactions

内容及储存

3´ RACE System components are listed in Table 1. Store the system at -20°C. Guaranteed stable for 6 months when properly stored.

Figures

手册

手册和实验方案

• 3′ RACE System for Rapid Amplification of cDNA Ends

• 3´ RACE System for Rapid Amplification of cDNA Ends

• 
  

化学品安全技术说明书(MSDS)

• 50119

• 51869

• y00122

• Y01138

• Y01420

• Y01421

• Y01424

• Y01425

• Y01426

• y02028

• y02222

• Y02226

• 
  

ss

引用及参考文献

Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA.

Flatz L, Bergthaler A, de la Torre JC, Pinschewer DD, 
Proc Natl Acad Sci U S A (2006) 103:4663-4668
Product usage: The recovery of infectious lymphocytic choriomeningitis virus from cDNA is presented in this article. The virus was produced in BHK-21 cells by co-transfecting plasmids carrying the viral genes with Lipofectamine. For the analysis of genetic viral tags, total RNA was extracted fr

---

Molecular characterization of the starfish inositol 1,4,5-trisphosphate receptor and its role during oocyte maturation and fertilization.

Iwasaki Hirohide; Chiba Kazuyoshi; Uchiyama Tsuyoshi; Yoshikawa Fumio; Suzuki Fumiko; Ikeda Masako; Furuichi Teiichi; Mikoshiba Katsuhiko; 
J Biol Chem (2002) 277:2763-2835
Product usage: Reverse transcription was performed using SuperScript II. PCR was performed and products were subcloned into the TA cloning vector, pCR2.1. Additional products were amplified using Platinum Pfx for subcloning. 5'- and 3'-untranslated region were isolated with the 5'-RACE System

---

Misexpression of the eyes absent family triggers the apoptotic program.

Clark S Wesley; Fee Brian E; Cleveland John L; 
J Biol Chem (2002) 277:3560-3567
Product usage: To acquire a complete cDNA, 5'-RACE and Superscript II was employed using mRNA from the human neuroblastoma cell lines LA-N-5 and CHP-134. To extend the 3'-end of the murine cDNA, 3'-RACE was performed.