5' RACE System for Rapid Amplification of cDNA Ends, Version 2.0

产品描述

描述

2.0版5´ RACE系统适用于cDNA末端的快速扩增 (RACE) (1) 及mRNA中确定点和5´ 末端之间的锚式PCR (2)。该系统可用于扩增序列信息较少的罕见mRNA，且可用于捕获mRNA的5´ 末端信息 (3,4)。5´ RACE系统：

• 利用SuperScript® II RNase H- RT的先进性能，提高第一链cDNA产量，增加cDNA长度

• 在一个反应中，利用至多1 µg的总RNA或poly(A)+ RNA制备特定的cDNA

• 提供更完善的第一链cDNA纯化步骤，获得适合加尾的cDNA

• 在与PCR相容的缓冲液中，以优化的反应条件使用重组TdT，为PCR提供高效的引物退火位点 (图1)

规格

Product Size:	10 reactions

内容及储存

5´ RACE系统包含的成分如表1所示。将试剂置于-20°C保存，DNA纯化组分置于+4°C保存。所有试剂正确储存时可稳定保存6个月。

Figures

手册

手册和实验方案

• 5′ RACE System for Rapid Amplification of cDNA Ends, Version 2.0

• 5´ RACE System for Rapid Amplification of cDNA Ends

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化学品安全技术说明书(MSDS)

• 50119

• 50354

• 50355

• 50356

• 50357

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• 50359

• y00122

• Y01138

• Y01320

• Y01420

• Y01426

• Y02003

• Y02007

• Y02008

• y02028

• y02222

• Y02223

引用及参考文献

The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia.

Touz Maria C.; Nores Maria J.; Slavin Ileana; Carmona Carlos; Conrad John T.; Mowatt Michael R.; Nash Theodore E.; Coronel Carlos E.; Lujan Hugo D.; 
J Biol Chem (2002) 277:8474-8481
Product usage: Giardia RNA for CWP1 and CWP2 were reverse transcribed. 5'-Rapid amplification of cDNA ends was performed and the primers 5'-CAGGGATTACAACATTTCG-3' and 5'-TCGTTCTTCTTAACTGG-3' to identify the 5' end sequence.

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Molecular characterization and role of bovine upstream stimulatory factor 1 and 2 in the regulation of the prostaglandin G/H synthase-2 promoter in granulosa cells.

Sayasith K, Bouchard N, Sawadogo M, Lussier JG, Sirois J, 
J Biol Chem (2004) 279:6327-6336
Product usage: To obtain additional sequences at the 5'-end of bovine USF2, the 5'-RACE system version 2.0 was used. Reverse transcription was performed as directed using antisense primer 13 and 5 µg of RNA from a preovulatory follicle. The first 5'-RACE/PCR reaction was performed with sense ab

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A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species.

Allander T; Emerson S U; Engle R E; Purcell R H; Bukh J; 
Proc Natl Acad Sci U S A (2001) 98:11609-11623
Product usage: RNA was extracted from the 50 µl of serum aliquots with Trizol reagent. A combination of methods was applied to obtain additional fragments covering the virus genome: PCR walking, subsequent identification of one additional clone by RDA, and bridging of the fragments by PCR, as