ThermoScript™ RT-PCR System for First-Strand cDNA Synthesis

货号	单位规格	目录价格 (CNY)	数量
	11146-016	100 reactions	9,910.00
	11146-024	25 reactions	2,919.00

产品概述

描述

ThermoScript™ Reverse Transcriptase (RT) 是鸟类 RT，RNase H 活性降低，设计用于具有高级结构，处理难度大的 RNA 模板的 RT-PCR。 ThermoScript™ RT-PCR 系统设计用于复制具有二级结构的 RNA 并改善引发的特异性。 与 PCR 结合使用时，该系统可用于检测稀有信使或者对来自少量细胞的特异性 mRNA 进行定量。 该系统含有 ThermoScript™ RT，克隆的鸟类反转录酶，这种酶经过改造，具有降低的 RNase H 活性和很高的热稳定性（最高 70°C）。 每个系统针对从总 RNA 或多聚 (A)+ RNA 产生第一链 cDNA 进行了优化 (1)（图 1）。 ThermoScript™ RT-PCR 系统：

• 针对 RT-PCR 进行了优化

• 可用于具有高级结构的 RNA 或基因特异性引物

• RNase H 活性降低，第一链 cDNA 产量更高

• 直接扩增第一链 cDNA 产物，无需有机萃取或沉淀

• 包含寡 (dT) 和随机六核苷酸以引发 cDNA 合成，用途灵活

• 可选择 Platinum® Taq DNA 聚合酶，在扩增最长达 3 kb 的模板时提供更高的特异性 (2)（图 2），或者 Platinum® Taq DNA 高保真聚合酶 (Platinum® Taq DNA Polymerase High Fidelity)，以扩增最长达 12 kb 的模板（图 3）

规格

Reverse Transcriptase:	ThermoScript™
Downstream Application:	Reverse Transcriptase PCR (RT-PCR)
Reaction Format:	Separate Components
Primer Type:	Oligo dT Primers, Random Primers
Product Size:	25 reactions
Shipping Condition:	Dry Ice
Optimal Reaction Temperature:	50-65°C

内容及储存

ThermoScript™ RT-PCR Systems are supplied with the components listed in Table 1. Store at -70°C. Guaranteed stable for 6 months when properly stored.

手册

文档

手册和实验方案

• ThermoScript RT-PCR System

化学品安全技术说明书(MSDS)

• 51537

• 51654

• 51655

• 51656

• y00122

• Y01138

• Y01203

• Y01220

• Y01420

• 引用及参考文献

• Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia.

  Rawat VP, Cusan M, Deshpande A, Hiddemann W, Quintanilla-Martinez L, Humphries RK, Bohlander SK, Feuring-Buske M, Buske C, 
  Proc Natl Acad Sci U S A (2004) 101:817-822
  Product usage: First-strand cDNA was synthesized from 1 µg of total RNA by using the ThermoScript RT-PCR system. A PCR product was cloned in frame to the 3' end of the histidine epitope of pCDNA6/ His A plasmid.

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  The in vivo role of p38 MAP kinases in cardiac remodeling and restrictive cardiomyopathy.

  Liao P; Georgakopoulos D; Kovacs A; Zheng M; Lerner D; Pu H; Saffitz J; Chien K; Xiao R P; Kass D A; Wang Y; 
  Proc Natl Acad Sci U S A (2001) 98:12283-12291
  Product usage: RNA samples were prepared from the heart tissues by using Trizol reagent (Life Technologies, Rockville, MD) according to the manufacturer's recommended protocol. The RNA dot-blot analysis was performed based on a published protocol using a set of oligonucleotide probes. Reverse t

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  A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells.

  Holt Jeffrey R; Gillespie Susan K H; Provance D William; Shah Kavita; Shokat Kevan M; Corey David P; Mercer John A; Gillespie Peter G; 
  Cell (2002) 108:371-452
  Product usage: We purified total RNA from utricles of genotyped mice, synthesized cDNA using oligo(dT)-containing primers, and carried out PCR (ThermoScript RT-PCR, Gibco-BRL).