SuperScript® VILO™ cDNA Synthesis Kit

产品概述

描述

The SuperScript® VILO™ cDNA Synthesis Kit provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). VILO is an acronym for variable input, linear output; and the SuperScript®: VILO™ cDNA Synthesis Kit provides the most consistent results for your RT experiments, with unprecedented linearity across the broadest range of input material.

Using the SuperScript® VILO™ cDNA Synthesis Kit
This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript® Enzyme Mix includes SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.

SuperScript® III RT
SuperScript® III RT is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT™ Recombinant Ribonuclease Inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination.

Optimized buffer
The 5X VILO™ Reaction Mix includes random primers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR.

规格

Reverse Transcriptase:	SuperScript® III
Downstream Application:	Real-Time Quantitative PCR (qPCR)
Reaction Format:	SuperMix or Master Mix
Sample Type (General):	RNA
Primer Type:	Random Primers
Product Size:	250 x 20 µL reactions
Shipping Condition:	Dry Ice
Optimal Reaction Temperature:	42° C

内容及储存

The SuperScript® VILO™ cDNA Synthesis Kit is supplied with 5X VILO™ Reaction Mix (1000 µL) and a 10X SuperScript® III Enzyme Blend (500 µL), which contains: SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.

Store at -20°C upon receipt.

手册

文档

手册和实验方案

• Product Info Sheet: SuperScript VILO cDNA Synthesis Kit

• Manual: Superscript VILO cDNA synthesis kit 50 and 250 (Japanese)

化学品安全技术说明书(MSDS)

• 100002278

• 100002280

引用及参考文献

Decrease of miR-146b-5p in monocytes during obesity is

引用及参考文献

associated 

with loss of the anti-

引用及参考文献

Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.

Hulsmans M, Van Dooren E, Mathieu C, Holvoet P, 
PLoS One (2012) 7:e32794-e32794
Product usage: General Oxidative Stress, To detect mitochondrial and intracellular ROS (mROS and iROS respectively) formation in treated THP-1 cells, measurements of MitoSOX Red and CellROX Deep Red fluorescence were performed by flow cytometry.

---

T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.

Heuts F, Rottenberg ME, Salamon D, Rasul E, Adori M, Klein G, Klein E, Nagy N, 
J Virol (2014) 88:3235-3245
Product usage: flow cytometry qdot antibody