SuperScript® Choice System for cDNA Synthesis

产品描述

描述

The SuperScript® Choice System is designed for synthesis of double-stranded cDNA from a purified mRNA population. Advantages of the SuperScript® Choice system include:

• The advanced capabilities of SuperScript® II RT resulting in greater cDNA yields and increased full-length cDNAs (1,3)
• Choice of primers for first-strand synthesis (2)
• One-tube format improving cDNA yield (4,5)
• cDNA is size-fractionated using an efficient and convenient pre-packed column
• One reaction converts up to 5 µg of mRNA into size-fractionated, EcoR I-adapted cDNA, ready for ligation into any EcoR I-digested vector

Performance and Quality Testing: Yields of the first- and secondstrand cDNA synthesis reactions and percentage of full-length double-stranded cDNA are assessed using the control RNA.

规格

Reverse Transcriptase:	SuperScript® II
Downstream Application:	cDNA Library Construction
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	3 reactions
Final Product:	cDNA (Second-Strand)
Optimal Reaction Temperature:	42° C

内容及储存

The SuperScript® Choice System for cDNA Synthesis includes all components listed in the table. Store at -20°C, except for cDNA size fractionation columns which are stored at +4°C.

手册

文档

手册和实验方案

• SuperScript Choice System for cDNA Synthesis

产品文献

• SuperScript II RT Brochure

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化学品安全技术说明书(MSDS)

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引用及参考文献

Pemphigus vulgaris IgG and methylprednisolone exhibit reciprocal effects on keratinocytes.

Nguyen VT, Arredondo J, Chernyavsky AI, Kitajima Y, Pittelkow M, Grando SA, 
J Biol Chem (2004) 279:2135-2146
Product usage: Double-strand cDNA was synthesized using the SuperScript Choice system from isolated keratinocyte mRNA.

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Mediation by a CREB Family Transcription Factor of NGF-Dependent Survival of Sympathetic Neurons

Antonella Riccio, 1 Sohyun Ahn, 1 Christopher M. Davenport, 1 Julie A. Blendy, 2 David D. Ginty 1* 
Science (1999) 286:2358-2361
Product usage: Messenger RNA was isolated from PC12 cells and primary neuronal cells with Microfast Track Kit (Invitrogen), and double-strand cDNA was obtained with the Superscript Choice System (Gibco). Reverse Northern analysis of Bcl-2 and GAPDH was performed.

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Blocking the secretion of hepatic very low density lipoproteins renders the liver more susceptible to toxin-induced injury.

Björkegren Johan; Beigneux Anne; Bergo Martin O; Maher Jacquelyn J; Young Stephen G; 
J Biol Chem (2002) 277:5476-5559
Product usage: Total RNA was isolated from liver biopsies with TRizol Reagent and cDNA was synthesized from the RNA with the Superscript Choice System.