Cloned AMV First-Strand cDNA Synthesis Kit

产品概述

描述

The Cloned AMV First-Strand Synthesis Kit combines the high performance of Invitrogen™ Cloned AMV RT with the convenience of optimized, pre-qualified reagents. The and ! subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen™ Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum® TaqDNA Polymerase for high-specificity PCR or with Platinum® Taq DNA Polymerase High Fidelity for subsequent PCR cloning.
The Cloned AMV First-Strand cDNA Synthesis Kit:

• Uses high-purity Cloned AMV for higher yields
• Includes 5X first-strand buffer with proprietary agents for more robust performance
• Replaces the cDNA Cycle® Kit and AMV Reverse

Transcriptase System
• Detects transcripts in 1.0 pg of RNA (Figure 1)
• Detects various mRNA targets ranging in size from 1.6 kb to 9.4 kb (Figure 2)

规格

Reverse Transcriptase:	AMV (Recombinant)
Downstream Application:	Reverse Transcriptase PCR (RT-PCR)
Reaction Format:	Separate Components
Form:	Frozen
Format:	Tube(s)
Quantity:	375 units
Template:	ssRNA
Sensitivity:	Low
Product Size:	25 reactions
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Dry Ice
Number of Reactions:	25 Reactions
Final Product Size(s):	9.4 kb or less
Optimal Reaction Temperature:	45-55° C

内容及储存

The Cloned AMV First-Strand cDNA Synthesis Kit is supplied with the components listed in Table 1. Store at -70°C. Guaranteed stable for 6 months when properly stored.

文档

手册和实验方案

• Cloned AMV First-Strand cDNA Synthesis Kit

产品文献

• Cloned AMV RT Brochrue

化学品安全技术说明书(MSDS)

• 12328032

• 50868

• y00122

• 引用及参考文献

• A Mammalian H+ Channel Generated Through Alternative Splicing of the NADPH Oxidase Homolog NOH-1

  Banfi B, Maturana A, Jaconi S, Arnaudeau S, Laforge T, Sinha B, Ligeti E, Demaurex N, Krause KH
  Science (2000) 287:138-142
  Product usage: Polyadenylated RNA was purified with the Fast Track kit from CaCo-2, dimethyl sulfoxide DMSO)-differentiated HL-60 cells, human peripheral blood leukocytes, and HEK-293 cells. First-strand cDNA was generated using AMV reverse transcriptase with random primers.