SuperScript® Plasmid System for cDNA Synthesis and Plasmid Cloning with Gateway™ Technology

货号	单位规格	目录价格 (CNY)	数量
	18248-013	3 reactions	15,560.

产品概述

SuperScript® 质粒系统设计用于从纯化的 mRNA 群合成双链 cDNA。 cDNA 适合通过定向克隆导入 pSPORT1 载体或 pCMV•SPORT6 质粒载体中，随后可生成 cDNA 文库并使用 Gateway® 技术。 SuperScript® 质粒系统：

• cDNA 产量更大，并且用 SuperScript® II RT 增加全长 cDNA

• 为定向克隆提供简化的引物-接头策略

• 为第一和第二链反应提供一管模式，提高 cDNA 产量

• 使用高效方便的预填充柱分离 cDNA

• 将插入片段连接进预先剪切的 Not I-Sal I 载体，这是为最大限度降低背景而制备的载体。

规格

Product Size:	3 reactions

内容及储存

The SuperScript® Plasmid System with Gateway® Technology includes components listed above. Store all cDNA cloning and ligation reagents at -20°C. Store cDNA size fractionation columns at +4°C.

Figures

Table 1

Outline of Procedure for SuperScript Lambda or Plasmid System

手册

文档

手册和实验方案

• SuperScript Plasmid System with Gateway Technology for cDNA Synthesis & Cloning

产品文献

• SuperScript II RT Brochure

化学品安全技术说明书(MSDS)

• 53884

• 8248RT

• y00122

• Y01116

• Y01122

• Y01123

• Y01127

• Y01128

• Y01129

• Y01131

• Y01132

• Y01138

• Y01145

• Y01181

• Y01301

• Y01425

• Y01663

• Y01664

• Y02226

• Y02321

• 
  

引用及参考文献

Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy.

Gomes M D; Lecker S H; Jagoe R T; Navon A; Goldberg A L; 
Proc Natl Acad Sci U S A (2001) 98:14440-14445
Product usage: Oligonucleotide primers for the amplification of DNA fragments containing wild-type or mutant versions of the atrogin-1 gene were designed with flanking attB1 or attB2 sites for insertion into the GATEWAY donor vector pDONR201. The PCR products were cloned directly into pDONR201

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A Mosquito Salivary Protein Inhibits Activation of the Plasma Contact System by Binding to Factor XII and High Molecular Weight Kininogen.

Isawa Haruhiko; Yuda Masao; Orito Yuki; Chinzei Yasuo; 
J Biol Chem (2002) 277:27651-27659
Product usage: A cDNA library was constructed from this mRNA using SuperScript plasmid system (Invitrogen) according to the manufacturer's inst12011061ructions.

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FEEL-1, a Novel Scavenger Receptor with in Vitro Bacteria-binding and Angiogenesis-modulating Activities.

Adachi Hideki; Tsujimoto Masafumi; 
J Biol Chem (2002) 277:34264-34334
Product usage: cDNA library construction,transient COS-1 and CHO-K1 cell transfection,clone FEEL-2 cDNA.

技术参数

• Regulatory Statement

 

• For Research Use Only. Not for use in diagnostic procedures. 

相关应用

cDNA Libraries & Library Construction