Dynabeads® Protein A for Immunoprecipitation

描述

Dynabeads® A 蛋白 (Dynabeads® Protein A) 为免疫沉淀提供了海藻糖和琼脂糖浆液之外的另一种选择。 Dynabeads® A 蛋白 (Dynabeads® Protein A) 在免疫沉淀中有以下作用：

• 将方案时间减少到 30 分钟。
• 显著降低非特异性结合造成的背景
• 保持蛋白质复合物的完整。对珍贵的蛋白质没有物理压力。

Dynabeads® A 蛋白广泛用于小规模 IgG 纯化和 Ab 标记、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 以及蛋白分离。 磁分离技术快速且温和，对靶蛋白的物理压力极小。 因此可以分离和浓缩易分解的成分，这些成分通常会在漫长的孵育过程中解离或被蛋白酶破坏。 天然蛋白质的构象和完整的大蛋白质复合体得以保留。

表面偶联有 A 蛋白的 Dynabeads® 可分离大多数哺乳动物免疫球蛋白 (Ig)。 参见下文图像与数据 (Images and Data) 中的结合强度表，查看不同种属和亚类的结合强度。 捕获的 Ig 量取决于起始样品中 Ig 的浓度以及 Ig 的类型和来源。

该产品在可重复性和自动化能力上秉承了 Dynal 的高标准，可提高分析的可靠性。

优点和特点： 

• 不使用柱子、不离心、没有耗时的样品预处理
• 小规模 IgG 纯化和免疫沉淀的完美选择
• 易于处理，方案简短
• 温和，对珍贵蛋白质的物理压力极小
• 微珠上的重组 A 蛋白不含白蛋白结合位点，避免了杂质蛋白质的共纯化

应用：
小规模 IgG 纯化、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 以及蛋白质分离。

结合能力：
捕获的 Ig 量取决于起始样品中 Ig 的浓度。 100 µl Dynabeads A 蛋白可从含有 20- 200 µg IgG ⁄ml 的样品中分离约 25- 30 µg 人 IgG。 绝大部分位点与 Fc 结合，实现最优的 Ig 取向

起始样品：
起始样品可以是唾液、血浆、腹水、血清和组织培养物或者杂交瘤上清。

其它信息：Dynabeads® A 蛋白和 G 蛋白还可以 OEM 形式提供。

Figures

Immunoprecipitation Reproducibility and Yield Comparison

Compare consistency of IP results for multiple users

Binding strengths
Protein G and Protein A differ in their binding strength to antibodies/ immunoglobulins (Ig) from different species and subclasses.

Comparison of results for all methods vs. Dynabeads® Protein A

Comparison of results for all methods vs. Dynabeads® Protein G

规格

Ligand Type:	Protein A
Form:	Beads in Suspension
Shelf Life:	24 months from date of manufacture
Product Size:	1 mL
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Room Temperature

内容及储存

Store these beads at 2-8°C.

手册和实验方案

• Dynabeads Protein A

• Immunoprecipitation using Protein A - 10006A

• Immunoprecipitation with Dynabeads® Protein A

产品文献

• Immunoprecipitation

• Proteomics - Looking to Improve Your Proteomic Workflow?

• Dynabeads® for protein complex isolation

• Immunoprecipitation Application Note

化学品安全技术说明书(MSDS)

• Dynabeads® Protein A for Immunoprecipitation

常见问题解答

What are Dynabeads® magnetic beads?

浏览答案r

---

What are the advantages of using Dynabeads® Protein A magnetic beads and Dynabeads® Protein G magnetic beads?

浏览答案r

---

What is the average shelf life of Dynabeads® magnetic beads?

浏览答案r

---

显示更多 ›

引用及参考文献

Resolving cadherin interactions and binding cooperativity at the single-molecule level.

Zhang Y, Sivasankar S, Nelson WJ, Chu S, 
Proc Natl Acad Sci U S A (2009) 106:109-114
Product usage: Labeling of E-cadherin for analysis of oligomerization by single molecule fluorescence spectrosocopy.

---

Regulation of Op18 during spindle assembly in Xenopus egg extracts.

Budde PP, Kumagai A, Dunphy WG, Heald R, 
J Cell Biol (2001) 153:149-158
Product usage: Dynabeads M-280 Streptavidin were coated with long fragments of biotinylated DNA using the Dynabeads Kilobase Binder kit. The DNA coated Dynabeads were used in ChIP assay and kinase assay. Dynabeads Protein A were subsequently coated with anti-Plx1 antibody and used for immunodep

---

Dynamic changes in transcription factor complexes during erythroid differentiation revealed by quantitative proteomics.

Brand M, Ranish JA, Kummer NT, Hamilton J, Igarashi K, Francastel C, Chi TH, Crabtree GR, Aebersold R, Groudine M, 
Nat Struct Mol Biol (2004) 11:73-80
Product usage: Dynabeads Protein A and -G were used for Chromatin Immunoprecipitation (ChIP). Briefly, MEL cells were crosslinked for 30 min at room temperature with 1% (w/w) formaldehyde. Antibodies were coupled to Dynabeads Protein A (for rabbit antibodies) or to Dynabeads Protein G (for goat

---

显示更多 ›