Dynabeads® Protein G for Immunoprecipitation

描述

Dynabeads® 蛋白G是一种极佳的海藻糖和琼脂糖浆液替代物，可用于免疫沉淀。在免疫沉淀中，Dynabeads® 蛋白G：

• 将实验方案时间缩短至30分钟。

• 显著降低了非特异性结合造成的背景

• 保持蛋白质复合物的完整性，对珍贵的蛋白质无物理压力。

Dynabeads® 蛋白G广泛应用于小规模IgG纯化和抗体标记、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 及蛋白质分离。磁性分离技术快速且温和，对靶蛋白的物理压力极小。因此可用于分离和浓缩易分解的成分，这些成分通常会在漫长的孵育过程中解离或被蛋白酶破坏。天然蛋白质的构象和完整的大蛋白复合体将受到保护。

表面偶联有蛋白G的Dynabeads® 可分离大多数哺乳动物免疫球蛋白 (Ig)。参见下文图像和数据中的结合强度表，查看不同种属和亚类的结合强度。捕获的Ig量取决于起始样品中Ig的浓度以及Ig的类型和来源。

该产品在可重复性和自动化能力方面秉承了Dynal的高标准，为您的分析提供了可靠性。

优点和特点：

• 不使用离心柱，无需离心或耗时的样品预处理

• 小规模IgG纯化和免疫沉淀的完美选择

• 方便的处理和简短的实验方案

• 温和，对珍贵的蛋白质的物理压力极小

• 微珠上的重组蛋白G不含有白蛋白结合位点，避免了残留蛋白质的共纯化

应用：

小规模IgG纯化、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 及蛋白质分离。

结合能力：

捕获的Ig量取决于起始样品中Ig的浓度。100 µl Dynabeads蛋白G可从含有20- 200 µg IgG/ml的样品中分离约25- 30 µg人IgG。绝大部分与Fc结合，可实现最佳Ig取向

起始样品：

起始样本可以是唾液、血浆、腹水、血清和组织培养物或杂交瘤上清液。

其它信息：Dynabeads® 蛋白A和蛋白G还可以OEM的方式提供。

Figures

Immunoprecipitation Reproducibility and Yield Comparison

Compare consistency of IP results for multiple users

Binding strengths
Protein G and Protein A differ in their binding strength to antibodies/ immunoglobulins (Ig) from different species and subclasses.

Comparison of results for all methods vs. Dynabeads® Protein A

Comparison of results for all methods vs. Dynabeads® Protein G

规格

Ligand Type:	Protein G
Form:	Beads in Suspension
Shelf Life:	24 months from date of manufacture
Product Size:	5 mL
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Room Temperature

内容及储存

将这些微珠置于2-8°C保存。

手册和实验方案

• Dynabeads Protein G

• Immunoprecipitation with Dynabeads® Protein G

产品文献

• Immunoprecipitation

• Dynabeads® for protein complex isolation

• Immunoprecipitation Application Note

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化学品安全技术说明书(MSDS)

• Dynabeads® Protein G for Immunoprecipitation

常见问题解答

How can I dissociate isolated proteins from Dynabeads® magnetic beads?

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What is the average shelf life of Dynabeads® magnetic beads?

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What are Dynabeads® magnetic beads?

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引用及参考文献

pLG72 modulates intracellular D-serine levels through its interaction with D-amino acid oxidase: effect on schizophrenia susceptibility.

Sacchi S, Bernasconi M, Martineau M, Mothet JP, Ruzzene M, Pilone MS, Pollegioni L, Molla G, 
J Biol Chem (2008) 283:22244-22256
Product usage: (1)D-Amino acid oxidase (DAAO) activity measurements on control and transfected U87 cell extracts (Amplex UltraRed reagent). (2) Immunostaining of human cortical slices and secondary astrocyte cultures. (3)Quantitative pulldown of DAAO complexes with pLG72 protein (Dynabeads).

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Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling.

Orba Y, Suzuki T, Makino Y, Kubota K, Tanaka S, Kimura T, Sawa H, 
J Biol Chem (2010) 285:1544-1554
Product usage: Analysis of cell cycle dependence of large T antigen (TAg) expression in human polyomavirus JC virus (JCV)-infected IMR-32 cells.

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Dynamic changes in transcription factor complexes during erythroid differentiation revealed by quantitative proteomics.

Brand M, Ranish JA, Kummer NT, Hamilton J, Igarashi K, Francastel C, Chi TH, Crabtree GR, Aebersold R, Groudine M, 
Nat Struct Mol Biol (2004) 11:73-80
Product usage: Dynabeads Protein A and -G were used for Chromatin Immunoprecipitation (ChIP). Briefly, MEL cells were crosslinked for 30 min at room temperature with 1% (w/w) formaldehyde. Antibodies were coupled to Dynabeads Protein A (for rabbit antibodies) or to Dynabeads Protein G (for goat

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