Dynabeads® Protein A Immunoprecipitation Kit

描述

This kit provides a faster and easier solution for your immunoprecipitation experiments than Sepharose or Agarose, and includes all reagents required to perform small scale immunoprecipitation (IP) using your own antibody (Ab) of choice. The kit is optimal for standard IP, as well as co-IP and ChIP. The kit contains the widely used Dynabeads® Protein A and include all required buffers for immunoprecipitation.

When using this kit, you will:

• Reduce your protocol time to <30 minutes.
• Decrease or even eliminate background caused by non-specific binding—Easily remove of all supernatant after incubation and washing, with no risk of sample loss
• Minimize antibody usage and maximize target protein recovery—Rapid binding kinetics and high capacity
• Keep your protein complexes intact—Gentle magnetic separation and rapid binding kinetics avoid physical stress on your precious proteins
• Improve reproducibility—Consistent high performance of the Dynabeads®
• Increase confidence in results—Optimised and reproducible buffer compositions

Applications:
• immunoprecipitation (IP),
• chromatin immunoprecipitation (ChIP),
• small scale antibody purification and other protein isolations.

The magnetic separation technology is fast and gentle, with no centrifugation, rapid binding kinetics and short protocol time. Hence your precious target proteins will experience minimal physical stress. This permits the isolation and concentration of labile complexes that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and intact, large protein complexes will be preserved.

Benefits and Features:
• Superior signal-to-noise properties
• Highly reproducible results
• No columns, centrifugations, or time-consuming pre-treatment of your samples
• Gentle, minimal physical stress on precious proteins
• Easy handling and simple, short protocol.

The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

Binding Capacity:
Approx. 250 µg hIgG per ml (30 mg) Dynabeads® Dynabeads® Protein A can be used with most antibodies (Ab). (See table for Binding Strength in the Images and Data section below for specific affinities of Ab from different species and subclasses). The amount of Ab captured is dependent on the Ab concentration in the starting sample and the type and source of the Ab.

Starting Samples:
Typically cell lysate, plasma, serum, tissue culture, hybridoma supernatants or similar.

Additional Information:
Dynabeads® Protein A and Dynabeads® Protein G are also available separately separately (without buffers), both as research products for end-users and in larger volumes for OEM supply.

Figures

Immunoprecipitation Reproducibility and Yield Comparison

Compare consistency of IP results for multiple users

Binding strengths
Protein G and Protein A differ in their binding strength to antibodies/ immunoglobulins (Ig) from different species and subclasses.

Comparison of results for all methods vs. Dynabeads® Protein A

Comparison of results for all methods vs. Dynabeads® Protein G

规格

Ligand Type:	Protein A
Form:	Bead
Ligand:	Protein A
Shelf Life:	24 months from date of manufacture
Product Size:	40 reactions
Bead Diameter:	2.8 µm
Concentration:	30 mg⁄ml
Sample Type (Specific):	Cell Extracts, Cell Cultures, Clinical Sample, Tissue Digests, Supernatants, Serum
High Throughput Compatibility:	High Throughput-Compatible
Shipping Condition:	Room Temperature

内容及储存

The kit contains 2 ml Dynabeads® Protein A (30 mg/ml), Antibody (Ab) Washing and Binding buffer, Washing Buffer and Elution Buffer.
Store at 2-8°C

手册和实验方案

• 100-06D Immunoprecipitation Kit Dynabeads Protein A

• Immunoprecipitation Protocol Using Dynabeads® Protein A or Dynabeads® Protein G

• Immunoprecipitation using Protein A - 10006D

产品文献

• Immunoprecipitation

• Immunoprecipitation Application Note

化学品安全技术说明书(MSDS)

• 10006D AB WASHING-BINDING BUFFER

• 10006D DYNABEADS PROTEIN A

• 10006D ELUTION BUFFER

• 10006D WASHING BUFFER

常见问题解答

What is the average shelf life of Dynabeads® magnetic beads?

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How can antibodies be coupled to Dynabeads® magnetic beads?

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What are the advantages of using Dynabeads® Protein A magnetic beads and Dynabeads® Protein G magnetic beads?

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