Sodium Pyruvate 100 mM Solution

描述

Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture medium is 1 mM.

Product Use
For in vitro diagnostic use.

Dual-site cGMP Manufacturing and Quality System
Gibco® Sodium Pyruvate Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® Sodium Pyruvate Solution product made in our Scotland facility (11360-039). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

规格

Product Size:	100 mL
Shipping Condition:	Room Temperature

内容及储存

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

手册和实验方案

• Transfecting Stealth™ RNAi or siRNA into 3T3 L1 Cells Using Lipofectamine® 2000

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      产品文献

• GIBCO® Media Bottles - Green Fact Sheet

化学品安全技术说明书(MSDS)

• Sodium Pyruvate (100 mM)

常见问题解答

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

浏览答案r

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引用及参考文献

A novel mechanism for regulating clonal propagation of mouse ES cells.

Ogawa K, Matsui H, Ohtsuka S, Niwa H, 
Genes Cells (2004) 9:471-477
Product usage: Products are used in the culture and maintenance of mouse embryonic stem cells under serum-free conditions.

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Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.

Shimizukawa R, Sakata A, Hirose M, Takahashi A, Iseki H, Liu Y, Kunita S, Sugiyama F, Yagami K, 
Genesis (2005) 42:47-52
Product usage: Products are used in the culture and maintenance of mouse embryonic stem cells under serum-free conditions.

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Polycystin-1, the product of the polycystic kidney disease 1 gene, co-localizes with desmosomes in MDCK cells.

Scheffers MS, van der Bent P, Prins F, Spruit L, Breuning MH, Litvinov SV, de Heer E, Peters DJ, 
Hum Mol Genet (2000) 9:2743-2750
Product usage: MDCK cells were cultured in DMEM/F12 medium supplemented with penicillin/streptomycin, GlutaMAX, 10mM HEPES, 1mM sodium pyruvate, and 10% FBS.

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