GlutaMAX™ Supplement

描述

Gibco® GlutaMAX™ supplement provides an alternative to L-Glutamine with improved stability and cell health. GlutaMAX™ supplement is suitable in both adherent and suspension culture of mammalian cells with no adaptation required. GlutaMAX™ supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ supplement is also available pre-supplemented in a variety of media formulations. Compared to L-Glutamine, GlutaMAX™ supplement:

• Minimizes toxic ammonia build-up

• Improves cell viability and growth

• Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-Glutamine, GlutaMAX™ supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-Glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-Glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ supplement that is not possible with L-Glutamine.1

Remains stable across a wide range of temperatures
GlutaMAX™ supplement is room-temperature stable and ready to use. GlutaMAX™ supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -20°C for convenient storage and handling. GlutaMAX™ supplement is even stable at 37°C for a week or more.1

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
GlutaMAX™ supplement is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® GlutaMAX™ supplement made in our Scotland facility (35050-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

1Gibco® GlutaMAX™ Supplement Media brochure, 2003.

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

规格

Glutamine:	GlutaMAX™-I
Form:	Liquid
pH Range:	4.7 - 6.0
Osmolality:	440 - 500 mOsm/kg
Concentrated:	100 X
Product Size:	100 mL
Classification:	Animal Origin-Free
Shipping Condition:	Room Temperature

手册和实验方案

• Product Insert: GlutaMAX I (100X) (Japanese)

• GlutaMAX-I (100X)

• Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons

• Culturing Human Neural Stem Cells

• Differentiating Neural Stem Cells into Neurons and Glial Cells

• Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes

• Cryopreservation and Recovery of Mature Differentiated Neural Cells

• Immunocytochemistry

• HepaRG™ Cell User Guide

• Thawing and Establishing Rat Glial Precursor Cells (GPCs)

• Creation of Embryoid Bodies from iPSCs using Complete KnockOut™ Serum Replacement Feeder-Free

• Feeder-Free Culture & Passaging of Human iPS Cells Using Complete KnockOut™ SR XenoFree Feeder-Free

        产品文献

• GlutaMAX™ Media brochure

• GlutaMAX™-I Media Supplement - Green Fact Sheet

• Advanced D-MEM and MEM

• Advanced D-MEM and MEM

• CD 293 Medium

• Human Embryonic Kidney (293) C

化学品安全技术说明书(MSDS)

• GlutaMAX™ Supplement

常见问题解答

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

浏览答案r

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引用及参考文献

Transcriptional regulation of the rat phospholipid translocase MDR2 gene expression by cholesterol synthesis inhibitors and sterol regulatory element-binding proteins

Hooiveld GJEJ, et al
P-glycoproteins and hepatobiliary secretion: studies on cloning, function, and expression (2000) -:105-118
Product usage: Primary rat hepatocytes were cultured and transfected in Williams Media E supplemented with GlutaMAX I. HepG2 cells were cultured and transfected in DMEM supplemented with GlutaMAX I.

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Efficient intracellular assembly of papillomaviral vectors.

Buck CB, Pastrana DV, Lowy DR, Schiller JT, 
J Virol (2004) 78:751-757
Product usage: Synthetic ORF encoding the BPV1 L1 protein was constructed with Invitrogen custom oligos purified with PAGE. Vector pUb6-V5-HisA was used to construct the target plasmids to express the ORF with Ecdysone inducible expression system. CV-1 and COS-7 cells were transfected using Lip

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A novel cytosolic dual specificity phosphatase, interacting with glucokinase, increases glucose phosphorylation rate.

Muñoz-Alonso MJ, Guillemain G, Kassis N, Girard J, Burnol AF, Leturque A, 
J Biol Chem (2000) 275:32406-32412
Product usage: The mhAT3F hepatoma cell line was grown on glass four-chamber slides in DMEM/F12 media supplemented with GlutaMAX, penicillin/streptomycin, 0.1uM insulin, 1uM dexamethasone, 1uM triiodothyronine, and 5% FBS.

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