L-Glutamine, 200 mM Solution

描述

Gibco® L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production. L-Glutamine, unlike most other amino acids, is not stable in solution. The rate at which this degradation proceeds is a function of time, temperature and pH. Life Technologies also offers Gibco® GlutaMAX™supplement as a stable alternative to L-glutamine.

Gibco® L-glutamine 200 mM Solution is a stock solution ready-to-use as a cell culture supplement. The optimal use concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 – 6 mM.

Product Use
For in vitro diagnostic use.

Dual-site cGMP Manufacturing and Quality System
Gibco® L-glutamine is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® L-glutamine product made in our Scotland facility (25030-024). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

规格

Glutamine:	L-Glutamine
Form:	Liquid
Format:	Bottle(s)
Concentrated:	100 X
Culture Type:	Adherent Cell Culture, Suspension Cell Culture
Product Size:	100 mL
Classification:	Animal Origin-Free
Volume (Metric):	100 ml
Phenol Red Indicator:	No Phenol Red
Shipping Condition:	Dry Ice

手册和实验方案

• Isolation and Digestion of Postnatal Primary Hippocampal and Cortical Neurons

• Isolation and Digestion of Prenatal Primary Hippocampal and Cortical Neurons

• Transfecting Plasmid DNA into ACHN Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into HCT-116 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into HEK 293 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into HT-29 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into MDA-MB-231-ATCC Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into MDA-MB-435 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into MOLT-4 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into NCI-H460 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into NTera2 Cells Using Lipofectamine® LTX Reagent

• Transfecting Plasmid DNA into SK-MEL-28 Cells Using Lipofectamine® LTX Reagent

             产品文献

• GIBCO® Media Bottles - Green Fact Sheet

• Advanced D-MEM and MEM

• Advanced D-MEM and MEM

• CD 293 Medium

• VP-SFM

化学品安全技术说明书(MSDS)

• L-Glutamine (200 mM)

常见问题解答

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

浏览答案r

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引用及参考文献

Optimized Nutrient Additives for Fed-Batch Cultures

Stephen F. Gorfien, William Paul, David Judd, Lia Tescione, and David W. Jayme
BioPharm International (2003) April:34-40
Product usage: Recombinant CHO cells engineered to overexpress b-galactosidase (9) or human rIgG (proprietary cell line) were rapidly adapted to growth in either CD CHO Medium (GIBCO, Invitrogen Corporation) supplemented with 8.0 mM glutamine or a modified CD CHO Medium prepared with 2.0 g/L gl

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Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.

Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S, 
Proc Natl Acad Sci U S A (2001) 98:3185-3190
Product usage: THP-1 cells were grown in RPMI medium supplemented with 10% FBS, 2mM L-glutamine, 1mM sodium pyruvate, 50uM 2-mercaptoethanol, 100 U/ml penicillin and 100 ug/ml streptomycin. COS-7 cells were grown in DMEM with glutaMAX, wupplemented with 10% FBS, 100 U/ml penicillin and 100 ug/

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Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.

Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO, 
J Biol Chem (2001) 276:24122-24128
Product usage: For purification of recombinant human wt protein C and protein C variants, cells were grown in OptiMEM with GlutaMAX medium.

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