TrypLE™ Express Enzyme (1X), no phenol red

描述

TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)

This TrypLE™ Express is modified as follows:

With	Without
• EDTA	• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

规格

Chelators:	EDTA
Phenol Red Indicator:	No Phenol Red
Classification:	Animal Origin-Free
Enzyme Stability:	Room Temperature Stable
Form:	Liquid
pH Range:	Check and Record
Osmolality:	Check and Record
Concentrated:	1 X
Product Size:	100 mL
Reagent Type:	Trypsin Replacement
Tests Performed:	In Vitro Bioassay
Shipping Condition:	Room Temperature

内容及储存

Storage conditions: 15°C to 30°C
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

培养基配方

TrypLE™ Express (1X), no phenol red

手册和实验方案

• Product Insert: TrypLE (Japanese)

• TrypLE Express - Japanese

• TrypLE Express - English

• Culturing Human Neural Stem Cells

• RNA Isolation and cDNA Preparation from Neural Stem Cells

• Dissociation of Cells from Primary Tissue

• Isolation, Primary Culture, and Cryopreservation of Human Neonatal Fibroblasts

   产品文献

• TrypLE™ Enzyme - Green Fact Sheet

• TrypLE Product News

• TrypLE™ Express: A Temperature Stable Replacement

• GIBCO Casepacks

        科研海报

• Proliferative & phenotypic characterization of human mesenchymal stem cells by flow cytometry & imag

化学品安全技术说明书(MSDS)

• TrypLE™ Express Enzyme (1X), no phenol red

常见问题解答

Do you have a non-animal-origin enzyme for cell dissociation?

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What is the difference between TrypLE™ Express and TrypLE™ Select?

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引用及参考文献

A rapid, cost-effective method for counting human embryonic stem cell numbers as clumps.

Prowse A, Wolvetang E, Gray P, 
Biotechniques (2009) 47:599-606
Product usage: (1)Fluorometric quantitation of human embryonic stem cells (hESC). (2) Analysis of hESC pluripotency markers by immunofluorescence and flow cytometry.

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A simple tool to improve pluripotent stem cell differentiation.

Chetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA, 
Nat Methods (2013) -