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描述
经过猪细小病毒和支原体实验测试。 包含 25 g/L 胰蛋白酶 (1:250) 和 8.5 g/L NaCl，但不含酚红。
来源：猪。
稀释： 在不含 Ca++ 和 Mg++ 的平衡盐溶液中无菌制备 1X 溶液
终浓度： 2.6 g 胰蛋白酶 (1:250)/L。
规格
Chelators: No EDTA Phenol Red Indicator: No Phenol Red Classification: Animal Origin Form: Liquid pH Range: 7.1 - 8.0 Osmolality: 270 - 320 mOsm/kg Concentrated: 10 X Product Size: 100 mL Reagent Type: Trypsin Tests Performed: In Vitro Bioassay Shipping Condition: Wet Ice 常见问题解答
How long can I keep trypsin in the refrigerator after thawing?引用及参考文献
Prokova Vassiliki; Mosialos George; Kardassis Dimitris;
J Biol Chem (2002) 277:9342-9392
Product usage: All reagents for cell culture (DMEM, fetal bovine serum, Trypsin-EDTA, phosphate-buffered saline, L-glutamine, and penicillin-streptomycin) were purchased from Invitrogen. This was for HEK-293, COS-7, and HepG2 cells which were maintained in Dulbecco's modified Eagle's medium (DMObukhov Alexander G; Nowycky Martha C;
J Biol Chem (2002) 277:16172-16180
Product usage: TRIzol Reagent was used to isolate total RNA from the mouse brain. The PCR product was subcloned into the pCR2.1 cloning vector using the Original TA cloning kit. Chromaffin cells as well as PC12 cells were transfected using LipofectAMINE 2000.Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A,
Nature (2011) 474:337-342
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