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描述
经过猪细小病毒和支原体实验测试。 HBSS (Hanks' balanced salt solutions) 中含 2.5 g/L 胰蛋白酶 (1:250) 和 0.38 g/L EDTA•4Na，不含 CaCl2、MgCl2 • 6H2O 和 MgSO4 • 7H2O。含酚红。
规格
Chelators: EDTA Phenol Red Indicator: Phenol Red Classification: Animal Origin Form: Liquid pH Range: 7.2 - 8.0 Osmolality: 270 - 320 mOsm/kg Concentrated: 1 X Product Size: 500 mL Reagent Type: Trypsin Tests Performed: In Vitro Bioassay Shipping Condition: Wet Ice 常见问题解答
How long can I keep trypsin in the refrigerator after thawing?引用及参考文献
Zabzdyr Jennifer L; Lillard Sheri J;
Anal Chem (2002) 74:1857-1862
Product usage: Gibco (Invitrogen) 0.25% Trypsin-EDTA was used to remove Cho-K1 cells from cell culture flasks. Gibco (Invitrogen) penicillin/streptomycin was present in the Cho-K1 cell growth medium to prevent bacterial growth. Gibco F-12K (Kaighn's modification) is optimal for growing Cho-K1Benbow U; Butticè G; Nagase H; Kurkinen M;
J Biol Chem (1996) 271:10715-10737
Product usage: ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methionSneddon JB, Borowiak M, Melton DA,
Nature (2012) 491:765-768
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