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描述
经过猪细小病毒和支原体实验测试。 在 Hanks 平衡盐溶液 (Hanks' Balanced Salt Solution) 中包含 2.5 g/L 胰蛋白酶 (1:250)，但不含 CaCl2、MgCl2 • 6H2O 和 MgSO4 • 7H2O。包含酚红。
来源：猪。
规格
Chelators: No EDTA Phenol Red Indicator: Phenol Red Classification: Animal Origin Form: Liquid pH Range: 7.2 - 8.0 Osmolality: 280 - 310 mOsm/kg Concentrated: 1 X Product Size: 500 mL Reagent Type: Trypsin Tests Performed: In Vitro Bioassay Shipping Condition: Wet Ice
常见问题解答
How long can I keep trypsin in the refrigerator after thawing?引用及参考文献
Kabos Peter; Kabosova Andrea; Neuman Toomas;
J Biol Chem (2002) 277:8763-8769
Product usage: Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized (0.02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min. Cell suspension was centrifuged at 400 r
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