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描述
经过猪细小病毒和支原体实验测试。 HBSS (Hanks' balanced salt solutions) 中含 0.5 g/L 胰蛋白酶 (1：250) 和 0.2 g/L EDTA•4Na，不含 CaCl2、MgCl2 • 6H2O 和 MgSO4 • 7H2O。含酚红。
规格
Chelators: EDTA Phenol Red Indicator: Phenol Red Classification: Animal Origin Form: Liquid pH Range: 7.2 - 8.0 Osmolality: 270 - 310 mOsm/kg Concentrated: 1 X Product Size: 500 mL Reagent Type: Trypsin Tests Performed: In Vitro Bioassay Shipping Condition: Wet Ice 常见问题解答
How long can I keep trypsin in the refrigerator after thawing?引用及参考文献
Prossnitz E R; Schreiber R E; Bokoch G M; Ye R D;
J Biol Chem (1995) 270:10686-10780
Product usage: Restriction enzymes and T4 DNA ligase from Invitrogen were used for cloning. Trypsin from Invitrogen was used for cell culture. For expression, the mutated FPR genes were subcloned into the EcoRI site of the vector pSFFV.neo, which contains the selectable marker aminoglycoside tBenbow U; Butticè G; Nagase H; Kurkinen M;
J Biol Chem (1996) 271:10715-10737
Product usage: ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methionNeville-Webbe HL, Coleman RE, Holen I,
Br J Cancer (2010) 102:1010-1017
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