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描述
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.
cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
规格
Color: Blue For Use With (Equipment): Light Microscope Sub-Cellular Localization: Cytoplasm & Cytosol Label or Dye: Trypan Blue Product Size: 100 mL Detection Method: Colorimetric Shipping Condition: Room Temperature
内容及储存
Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture手册和实验方案
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)
Cryopreservation and Recovery of Mature Differentiated Neural Cells
FreeStyle™ 293 Expression System - for large-scale transfection of suspension 293 cells in a
Isolation, Primary Culture, and Cryopreservation of Human Neonatal Fibroblasts
常见问题解答
How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?引用及参考文献
Degenhardt Kurt; Sundararajan Ramya; Lindsten Tullia; Thompson Craig; White Eileen;
J Biol Chem (2002) 277:14127-14161
Product usage: After 16 h of treatment cells were harvested by trypsinization, centrifuged, and resuspended in PBS. Cells were diluted 1:100 in 0.25% trypan blue solution (Invitrogen).Esumi Hiroyasu; Izuishi Kunihiko; Kato Kazuyoshi; Hashimoto Koichi; Kurashima Yukiko; Kishimoto Atsuhiro; Ogura Tsutomu; Ozawa Takayuki;
J Biol Chem (2002) 277:32791-32799
Product usage: All products referenced were used to subculture HepG2 and WI-38 cells. The G418 was used for selection of HepG2 cells transfected with certain expression vectors. G418 was used at a concentration of 640 ug/mL for 2 weeks.
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