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描述
The antibiotics Penicillin, Streptomycin, and Neomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified fromStreptomyces griseus and Neomycin from Streptomyces fradiae. Streptomycin and Neomycin act by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. We offer a variety of antibiotics and antimycoticsfor cell culture applications.
This solution contains 5 mg of penicillin, 5 mg of streptomycin, and 10 mg of neomycin per ml in 0.85% saline.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
规格
Validated Application: Prevention of Cell Culture Contamination Agent: Streptomycin, Penicillin, Neomycin Form: Liquid Reagent Type: Antibiotic Concentrated: 100 X Product Size: 100 mL Shipping Condition: Dry Ice
内容及储存
Store in freezer (-5 to -30°C).化学品安全技术说明书(MSDS)
引用及参考文献
Benbow U; Butticè G; Nagase H; Kurkinen M;
J Biol Chem (1996) 271:10715-10737
Product usage: ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methion
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