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描述
在 10 mM 柠檬酸盐缓冲液中含有 10000 单位的青霉素(碱)、10000 µg 的链霉素(碱)以及 29.2 mg L-谷氨酰胺/ml(0.85% 盐水),以保持青霉素效力。 使用青霉素 G(钠盐)和链霉素硫酸盐。
抗菌谱: 革兰氏阳性和革兰氏阴性菌。
规格
Validated Application: Prevention of Cell Culture Contamination Agent: L-Glutamine, Streptomycin, Penicillin Form: Liquid Reagent Type: Antibiotic, Supplement Concentrated: 100 X Product Size: 100 mL Concentration: 50 mg⁄mL Shipping Condition: Dry Ice 引用及参考文献
Su Hua Poo; Nakada-Tsukui Kumiko; Tosello-Trampont Annie-Carole; Li Yonghe; Bu Guojun; Henson Peter M; Ravichandran Kodimangalam S;
J Biol Chem (2002) 277:11772-11781
Product usage: COS-7 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 5% bovine calf serum, 5% fetal calf serum, and penicillin/streptomycin/glutamine.Messenger Moira M; Saulnier Ronald B; Gilchrist Andrew D; Diamond Phaedra; Gorbsky Gary J; Litchfield David W;
J Biol Chem (2002) 277:23054-23118
Product usage: Constructs encoding CK2 with a C-terminal HA tag were generated using pRc/CMV (Invitrogen). The PCR product (~490 bp) was ligated into PCR-Blunt (Invitrogen) and sequenced. U20S/UTA6 cells were grown in Dulbecco's modified Eagle's medium with 10% fetal calf serum, 0.1 mg/ml stWong Wai K; Ou Xiao-Ming; Chen Kevin; Shih Jean C;
J Biol Chem (2002) 277:22222-22252
Product usage: The HepG2 (human hepatocytoma) cell line was purchased from the American Type Culture Collection and grown in Dulbecco's modified Eagle's medium supplemented with 10 mM Hepes, 2 mM L-glutamine, 100 units/ml penicillin, 10 µg/ml streptomycin, and 10% fetal bovine serum (Invitrogen





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