当前位置: 首页 > 生化试剂 > Puromycin Dihydrochloride
描述
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 7.2–7.5) in 10 vials each containing 1 mL.
Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection
See recommendations for working concentrations for selection antibiotics.
Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
规格
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation Agent: Puromycin Form: Liquid Reagent Type: Antibiotic (Selective) Quantity: 10 vials Sterility: Sterile Product Size: 10 x 1 mL Concentration: 10 mg⁄ml Formula Weight: 544.43 g⁄mole Shipping Condition: Dry Ice
内容及储存
Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture化学品安全技术说明书(MSDS)
常见问题解答
Which of your antibiotics (Geneticin®, Zeocin™, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?
Global - English
China - 中文
Brasil - Português
France - Français
Germany - Deutsch
Hungary - Magyarország
Italy - Italiano
Japan -日本语
Russia - Россия
United States - English
