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描述
C17H26N8O5•HCl
式量： 458.9 克/摩尔
杀稻瘟菌素是一种来自灰色产色链霉素 (Streptomyces griseochromogenes) 的核苷类抗生素。 它在原核和真核细胞中都是强力翻译抑制剂。 来自土曲霉 (Aspergillus terreus) 的 bsd 基因的产物能够提供杀稻瘟菌素抗性。 杀稻瘟菌素具有快速而强效的作用模式，很低的抗生素浓度便能导致细胞迅速死亡。 大肠杆菌 (E. coli) 菌株通常对 50 µg/ml 的浓度敏感，而哺乳动物细胞对低至 2 到 10 µg/ml 的浓度敏感。 细胞迅速死亡，不到一周即可形成具有杀稻瘟菌素抗性的稳定哺乳动物细胞系，（图 1）。
杀稻瘟菌素和杀稻瘟菌素抗性基因 (bsd) 受美国专利 No.5,527,701 保护，以专利许可出售，仅用于研究目的。 如果要将其用于商业用途，请联系：8008208982或4008208982
Figures
Figure 1 - Time course of selection with blasticidin
规格
Validated Application: Bacterial Selection, Eukaryotic Selection⁄Stable Cell Line Generation Agent: Blasticidin Form: Powder Reagent Type: Antibiotic (Selective) Product Size: 50 mg
内容及储存
Blasticidin S HCl is supplied as a powder and should be stored at -20°C. Blasticidin is guaranteed stable for 6 months when properly stored.常见问题解答
Which of Invitrogen™'s antibiotics (Geneticin®, Zeocin™, Hygromycin, and Blasticidin) can be used together for selection in mammalian cells?What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?What solvent can be used to dissolve Blasticidin?
引用及参考文献
Yoshizuka N, Yoshizuka-Chadani Y, Krishnan V, Zeichner SL,
J Virol (2005) 79:11366-11381
Product usage: The amplified PCR product was subcloned in a plasmid with a tetracycline-responsive DNA element, pcDNA5/FRT/TO/TOPO. To establish a tetracycline-inducible system in 293 cells, pcDNA5/FRT/TO vectors encoding wild-type or mutant Vpr and pOG44 vector, which expresses Flp recombinasXia ZB, Popovic R, Chen J, Theisler C, Stuart T, Santillan DA, Erfurth F, Diaz MO, Zeleznik-Le NJ,
Proc Natl Acad Sci U S A (2005) 102:14028-14033
Product usage: Stable 293 and Jurkat cell lines inducibly expressing MLL and MLL-AF4 were established as follows. The Flp-In T-REx 293 cell line was used to cotransfect pOG44 along with either pcDNA5/FRT/TO [MLL-AF4, MLL(672), MLL(1250), and MLL] or pcDNA5/FRT/TO (vector alone). Stable clones wThomas H, Senkel S, Erdmann S, Arndt T, Turan G, Klein-Hitpass L, Ryffel GU,
Nucleic Acids Res (2004) 32:e150-e150
Product usage: The Flp-in T-Rex system was used to generate a Flp-in T-Rex INS-1 pancreatic ß-cell line with stable integration of an FRT site and the tetracycline repressor. This cell line was then used to conditionally express transcription factors HNF6, HNF4 and HNF1ß and microarray analysis
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