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描述
Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome.
Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 1–20 µg/mL depending on the cell line. This product is provided as a solution at 10 mg/mL in 20 mM HEPES (pH 7.2–7.5) and is supplied in 10 vials each containing 1 mL.
In addition, Blasticidin S is used as a bacterial selection antibiotic in the concentration range of 50–100 µg/mL. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD or bsr. These deaminases convert Blasticidin S to a nontoxic derivative. To maintain the activity of Blasticidin S for selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0.
Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection
See recommendations for working concentrations for selection antibiotics.
Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
规格
Validated Application: Bacterial Selection, Eukaryotic Selection⁄Stable Cell Line Generation Agent: Blasticidin Form: Liquid Reagent Type: Antibiotic (Selective) Quantity: 10 vials Sterility: Sterile Product Size: 10 x 1 mL Concentration: 10 mg⁄ml Formula Weight: 458.9 g⁄mole Shipping Condition: Dry Ice
内容及储存
Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Frozen
Shelf life: 9 months from date of manufacture手册和实验方案
常见问题解答
What is the optimal pH of low salt LB for LB + blasticidin plates?What is the mode of action on the following antibiotics: Blasticidin, Geneticin® (G418), Hygromycin, and Zeocin™?What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?
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