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描述
Zeocin™ 试剂是博来霉素抗生素家族的一个成员。 其抗性由编码一种 13,665 道尔顿蛋白质的 Sh ble 基因赋予。 在表达这种蛋白质的细胞中，Zeocin™ 无法结合和切割细胞 DNA。 Zeocin™ 对大多数好氧细胞有效，可用于选择哺乳动物和昆虫细胞系、酵母以及细菌。 选择细胞时，Zeocin™ 的用量为 50-2000 ug/ml（通常 300 ug/ml），具体取决于细胞类型。 化学式:
C55H83N19O21S2Cu
式量： 1137.41 克/摩尔
规格
Validated Application: Bacterial Selection, Eukaryotic Selection⁄Stable Cell Line Generation Agent: Zeocin™ Form: Liquid Reagent Type: Antibiotic (Selective) Product Size: 8 x 1.25 mL Concentration: 100 mg⁄ml
内容及储存
This reagent is supplied at a concentration of 100 mg⁄ml. Store at -20°C in the dark. Guaranteed stable for 6 months when properly stored.手册和实验方案
化学品安全技术说明书(MSDS)
常见问题解答
What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?What is the mode of action on the following antibiotics: Blasticidin, Geneticin® (G418), Hygromycin, and Zeocin™?If there are no zeocin-containing YPD plates readily available, would it be possible to spread zeocin on top of YPD plates and still retain efficient selection of yeast?
引用及参考文献
Rogers LM, Kim YK, Guo W, Gonzalez-Candelas L, Li D, Kolattukudy PE
Proc Natl Acad Sci U S A (2000) 97:9813-19131
Product usage: DH5Alpha cells were used for propagation of plasmids. pSV40/Zeo was used as a template for amplification of the Zeocin-resistance gene. Taq Polymerase was used for PCR-screening of spores. TRIzol was used for isolation of total RNA from plant tissue. SuperScript II was used for rPoon M, Liu B, Taubman MB
Mol Cell Biol (1999) 19:6471-6478
Product usage: Rat aortic SMC cells were transfected with the pVgRXR vector. Stable cell lines were selected with Zeocin. Constructs were ligated into pIND. Cells were induced with 10 uM ponasterone.Ali S, Pellerin C, Lamarre D, Kukolj G,
J Virol (2004) 78:491-501
Product usage: Zeocin-resistant Flp-In 293 cells (2.2 x 10e6) and Huh-7 cells (1 x 10e6) were maintained in DMEM-10% FBS containing zeocin (100 µg/ml) and neomycin (500 µg/ml) respectively. Cells were transfected with Lipofectamine 2000. 20 µg of RNA and 40 µl of transfection reagent were adde
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