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描述
晶体、未消毒（G418 硫酸盐）
稳定性：非常稳定，可以粉末形式在室温下 (15°C 至 30°C) 保存最多 3 年；可溶于水。
规格
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation Agent: Geneticin® (G-418) Form: Powder Reagent Type: Antibiotic (Selective) Product Size: 5 g Shipping Condition: Room Temperature
常见问题解答
Can Neomycin be used in mammalian selection? Can Neomycin be used instead of Kanamycin in bacterial selection?Which of Invitrogen™'s antibiotics (Geneticin®, Zeocin™, Hygromycin, and Blasticidin) can be used together for selection in mammalian cells?What is the mode of action on the following antibiotics: Blasticidin, Geneticin® (G418), Hygromycin, and Zeocin™?
引用及参考文献
Reuven NB, Antoku S, Weller SK,
J Virol (2004) 78:4599-4608
Product usage: Sf9 cells were grown in Grace's medium, D-MEM contained 5% FCS and pen-strep. The 6-5 cell line was maintained with G418. Restriction endonucleases, other DNA-modifying enzymes and DNA primers were purchased from Invitrogen. For preparation of a recombinant baculovirus expresBruel C, Cha K, Reeves PJ, Getmanova E, Khorana HG
Proc Natl Acad Sci U S A (2000) 97:3004-3009
Product usage: The rhodopsin kinase gene was cloned into pPIC3. This vector has since been discontinued (pPIC3.5K, an alternative, is a derivative of pPIC3). The mammalian expression vector pCMV5 conferred geneticin resistance.Deng Yibin; Lin Yahong; Wu Xiangwei;
Genes Dev (2002) 16:33-45
Product usage: Human colon cancer HCT116 Bax+/+ and Bax-/- cells were transfected with lipofectamine and transfected cells were harvested 24-48 h for transient analysis. Stable cell lines including those expressing Bcl-xl, GFP, GFP-Bax, GFP-Bax C21, GFP-Smac/DIABLO were derived by selecting tra
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