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描述
SYPRO® Ruby Protein Gel Stain is a highly sensitive, ready-to-use fluorescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). SYPRO® Ruby stain is ideal for use in 1D and 2D PAGE. The sensitivity of SYPRO® Ruby Gel Stain is as good as or better than the best silver staining techniques, and this stain offers several advantages over silver staining:
• Simple staining procedure—no destaining or timed steps required
• Linear quantitation range over three orders of magnitude
• Compatibility with mass spectrometry and microsequencing
Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO® Photographic Filter (Cat. No. S-6656) is recommended.
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规格
Target Molecule: Protein Label or Dye: SYPRO® Ruby Detection Method: Fluorescent Detection Location: In-Gel Detection Product Size: 200 mL Shipping Condition: Room Temperature
内容及储存
Store at room temperature and protect from light.荧光光谱
SYPRO Ruby protein gel stain
Fluorescence Ex/Em spectra of SYPRO® Ruby protein gel stain.
手册和实验方案
Molecular probes手册
化学品安全技术说明书(MSDS)
常见问题解答
Can proteins from 1D SDS-PAGE gels be transferred to PVDF or nitrocellulose after being stained with SYPRO™ Ruby protein gel stain?Is there a recommended protocol for SYPRO® Ruby Protein Gel Stain on NuPAGE®, Tris-Glycine, and Tricine gels?引用及参考文献
Hochgräfe F, Wolf C, Fuchs S, Liebeke M, Lalk M, Engelmann S, Hecker M,
J Bacteriol (2008) 190:4997-5008
Product usage: Labeling of S-nitrosylated and reversibly oxidized protein thiols.Monasky MM, Biesiadecki BJ, Janssen PM,
J Mol Cell Cardiol (2010) 48:1023-1028
Product usage: Assessment of myofilament calcium sensitivity and myofilament protein phosphorylation status in right ventricular trabeculae isolated from rabbit hearts.Di Michele M, Marcone S, Cicchillitti L, Della Corte A, Ferlini C, Scambia G, Donati MB, Rotilio D,
J Proteomics (2010) 73:879-898
Product usage: Characterization of the glycoproteome of the human ovarian cancer cell line A2780 and its paclitaxel resistant counterpart A2780TC1 by 2D gel electrophoresis followed by mass spectrometry.
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