SilverQuest™ Silver Staining Kit

描述

The SilverQuest™ Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. Silver staining allows detection of most proteins since it is 30-fold more sensitive than staining with colloidal Coomassie™ G-250. The SilverQuest™ Silver Staining Kit is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

• High sensitivity – Protein detection at sub-nanogram level
• Compatible – Staining compatible with mass spectrometry analysis
• Rapid – Protein bands can be visualized within an hour
• Easy to use – Color-coded solutions for easy staining protocol

The SilverQuest™ Silver Staining Kit is based on the chemical reduction of silver ions to metallic silver on a protein band. The protein bands are visualized as spots where reduction begins. For more details on the mechanism of silver staining, refer to Rabilloud et al, 1994.

Ideal for Rapid Preparation of Samples for Mass Spectrometry
The mass spectrometry problems associated with traditional silver stains are mitigated with the SilverQuest™ Silver Staining Kit because the reagents used do not modify protein side chains and do not interfere with trypsin digestion. Typically, in less than 90 minutes, 0.3 ng of protein can be detected (Figure 1). For added speed, an optimized microwave procedure can typically be completed in 30 minutes without compromising sensitivity.

Mass Spectrometry-Compatible Reagents
The SilverQuest™ Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest™ reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so as to leave lysine and arginine side-chains unmodified. Additionally, the SilverQuest™ destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction, and yield better coverage during mass spectrometry analysis.

Figures

Sub-nanogram sensitivity with the SilverQuest™ Silver Staining Kit.

规格

Target Molecule:	Protein
Label or Dye:	SilverQuest™ Silver Stain
Detection Method:	Colorimetric
Detection Location:	In-Gel Detection
Product Size:	1 kit

内容及储存

The SilverQuest™ Silver Staining Kit is supplied with sufficient sensitizer, stainer, developer, stop solution, and destaining solutions for 25 mini-gels. Store the kit at room temperature. Guaranteed stable for 6 months when properly stored.

手册和实验方案 

• SilverQuest Silver Staining Kit

• Silver Staining

  产品文献

• NuPAGE® Large Protein Kits and HiMark™ Protein Standards

• SilverQuest Silver Staining

化学品安全技术说明书(MSDS)

• 461161

• 461162

• 461163

• 461164

• 461165

• 461166

• 461167

常见问题解答

How much protein is required for mass spectrometry analysis by MALDI-TOF?

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If you need to stop in the middle of the SilverQuest™ or SilverXpress® silver staining procedure, at what point should this be done?

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What is the sensitivity of Coomassie staining and silver staining?

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引用及参考文献

The formation of highly soluble oligomers of alpha-synuclein is regulated by fatty acids and enhanced in Parkinson′s disease.

Sharon R, Bar-Joseph I, Frosch MP, Walsh DM, Hamilton JA, Selkoe DJ, 
Neuron (2003) 37:583-595
Product usage: For detection by Western blotting,75 ng protein was loaded, and for detection by silver staining (Invitrogen, Carlsbad, CA), 500 ng; these aliquots were from the same reaction. Benchmark prestained marker is used for electrophoresis.

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Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.

Takao-Rikitsu E, Mochida S, Inoue E, Deguchi-Tawarada M, Inoue M, Ohtsuka T, Takai Y, 
J Cell Biol (2004) 164:301-311
Product usage: For immunoprecipitation, proteins were extracted from the PSD fraction with 1% SDS, followed by dilution with 5 volumes of the Triton X-100 lysis buffer. Immunoprecipitation was followed by Western blotting and silver staining using the SilverQuest Kit.

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The R116C Mutation in alpha A-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis.

Andley Usha P; Patel Harendra C; Xi Jing-Hua; 
J Biol Chem (2002) 277:10178-10264
Product usage: HLE B-3 cells were lysed for 30 min on ice with immune precipitation buffer. Supernatants were treated with a primary antibody to A-crystallin and immunoprecipitated with Protein A/G-agarose beads. Immunoprecipitates were washed three times, resuspended in SDS-PAGE sample buffer,

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