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描述
MagicMark™XP Western蛋白标准品专门设计用于直接在Western印迹膜上简单方便地估测蛋白分子量。该标准品由9种重组蛋白组成,每种蛋白均有一个IgG结合位点。IgG结合位点可与检测靶蛋白的一抗或二抗结合,进而可在Western 印迹膜中直接观察标准品。MagicMark™XP 标准品可与所有的western试剂盒和底物相兼容(化学发光、直接显色和荧光检测),并提供:
•采用常用的免疫检测方法,在Western 印迹膜上直接对分子量标准条带进行显影
•从分子量20到220间按等量增加,有9个清晰条带
•方便、即用型——无需混合、还原和加热
若有需要,这些条带可用Coomassie或转渍膜染剂显影
仅限科研使用。不可用于人或动物的治疗或诊断。
Figures
Clear bands visualized with Coomassie® stain on SDS-PAGE gel.
Sharp bands and sensitive detection using various western detection systems.
规格
Gel Compatibility: NuPAGE® Bis-Tris Gels, Novex® Tris-Glycine Gels, Novex® Tricine Gels, NuPAGE® Tris-Acetate Gels, Bolt® Bis-Tris Plus Gels, SDS-PAGE Gels Stain Type: Unstained Size Range: 20 to 220 kDa System: SDS-PAGE Product Size: 250 µL Shipping Condition: Dry Ice
内容及储存
250 µl of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue. Store at -20°C.化学品安全技术说明书(MSDS)
分析证书(COA)
常见问题解答
What can I use as a positive control or marker on my western blots?What is the composition of the MagicMark™ XP Western Protein Standard?引用及参考文献
Molecular weight estimation of proteins by gel electrophoresis revisitedMehrnoosh Sadeghi, Mahbod Hajivandi, Roumen Bogoev, and Joseph Amshey
Focus (2003) 25:35-39
Product usage: Estimation of molecular weight using unstained protein standards







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