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  • MagicMark™ XP Western Protein Standard (20-220 kDa)

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描述

MagicMark™XP Western蛋白标准品专门设计用于直接在Western印迹膜上简单方便地估测蛋白分子量。该标准品由9种重组蛋白组成,每种蛋白均有一个IgG结合位点。IgG结合位点可与检测靶蛋白的一抗或二抗结合,进而可在Western 印迹膜中直接观察标准品。MagicMark™XP 标准品可与所有的western试剂盒和底物相兼容(化学发光、直接显色和荧光检测),并提供:

•采用常用的免疫检测方法,在Western 印迹膜上直接对分子量标准条带进行显影
•从分子量20到220间按等量增加,有9个清晰条带
•方便、即用型——无需混合、还原和加热

若有需要,这些条带可用Coomassie或转渍膜染剂显影

仅限科研使用。不可用于人或动物的治疗或诊断。


Figures

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Clear bands visualized with Coomassie® stain on SDS-PAGE gel.



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Sharp bands and sensitive detection using various western detection systems.


规格

Gel Compatibility:NuPAGE® Bis-Tris Gels, Novex® Tris-Glycine Gels, Novex® Tricine Gels, NuPAGE® Tris-Acetate Gels, Bolt® Bis-Tris Plus Gels, SDS-PAGE Gels
Stain Type:Unstained
Size Range:20 to 220 kDa
System:SDS-PAGE
Product Size:250 µL
Shipping Condition:Dry Ice


内容及储存

250 µl of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue. Store at -20°C.

常见问题解答

What can I use as a positive control or marker on my western blots?

What is the composition of the MagicMark™ XP Western Protein Standard?

引用及参考文献

Molecular weight estimation of proteins by gel electrophoresis revisited
Mehrnoosh Sadeghi, Mahbod Hajivandi, Roumen Bogoev, and Joseph Amshey
Focus (2003) 25:35-39
Product usage: Estimation of molecular weight using unstained protein standards


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