High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

描述

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

规格

Reverse Transcriptase:	MultiScribe™ MuLV
Downstream Application:	Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	1,000 reactions
Final Product:	cDNA (First-Strand)
Final Product Size(s):	7 kb or less
Optimal Reaction Temperature:	37° C

内容及储存

Contents:
• 10X RT Buffer (2 × 1 mL)
• 10X RT Random Primers (2 × 1 mL)
• 25X dNTP Mix (100 mM, 1 mL)
• MultiScribe® Reverse Transcriptase (1 mL at 50 U/µL)
• RNase Inhibitor (10 × 0.1 mL at 20 U/µL)

Store between -15°C and -25°C.

手册和实验方案

• Protocol: High-Capacity cDNA Archive Kit: Protocol: Rev A (English )

• Protocol: High-Capacity cDNA Reverse Transcription Kits For 200 and 1000 Reactions (English )

• (Japanese) Quick Reference: High-Capacity cDNA Reverse Transcription Kits

         产品文献

• Product Bulletin: High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit): First Strand cDNA Synthesis Reverse Transcription Kit

化学品安全技术说明书(MSDS)

• 362271

• 4319979

• 4319981

• 4319983

• N8080119

常见问题解答

Can I order the RNase Inhibitor from the High-Capacity cDNA Reverse Transcription Kit separately?

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How long can I store the cDNA from my reverse transcription step?

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What is the difference between the High-Capacity RNA-to-cDNA™ Kit, the High-Capacity cDNA Reverse Transcription Kit, and the SuperScript® VILO™ Master Mix?

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引用及参考文献

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.

Mortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK, 
Proc Natl Acad Sci U S A (2010) 107:832-837
Product usage: Detection of mitochondrial depolarization (TMRM), superoxide generation (MitoSOX Red) and cardiolipin oxidation (nonyl acridine orange) in murine erythroid cells.

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Tyramide amplification allows anterograde tracing by horseradish peroxidase-conjugated lectins in conjunction with simultaneous immunohistochemistry

Kressel M
J Histochem Cytochem (1998) 46:527-533

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Grape seed proanthocyanidins reduce oxidant stress in cardiomyocytes.

Shao ZH, Qin YM, Becker LB, Hoek TL, Li CQ, Schumacker PT, Dey L, Wu JA, Xie JT, Yuan CS
Acad Emerg Med (2001) 8:562-562

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