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描述
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
Features of this kit include:
• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits
Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
规格
Reverse Transcriptase: MultiScribe™ MuLV Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR) Reaction Format: Separate Components Sample Type (General): RNA Product Size: 1,000 reactions Final Product: cDNA (First-Strand) Shipping Condition: Dry Ice Final Product Size(s): 7 kb or less Optimal Reaction Temperature: 37° C
内容及储存
Contents:
• 2 × 1 mL of 10X RT Buffer
• 2 × 1 mL of 10X RT Random Primers
• 1 × 1 mL of 25X dNTP Mix (100 mM)
• 1 × 1 mL of MultiScribe® Reverse Transcriptase (50 U/µL)
Store between -15°C and -25°C.手册和实验方案
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