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描述
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 µg of input total RNA in a single 20 µl reaction. Reactions can be scaled up to 100 µl to generate 10 µg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 µg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
规格
Reverse Transcriptase: MultiScribe™ MuLV Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR) Reaction Format: Separate Components Sample Type (General): RNA Product Size: 200 reactions Final Product: cDNA (First-Strand) Shipping Condition: Dry Ice Final Product Size(s): 7 kb or less Optimal Reaction Temperature: 37° C
内容及储存
10✕ RT Buffer, 10✕ RT Random Primers, 25✕ dNTP Mix (100 mM), and MultiScribe® Reverse Transcriptase are included and should be stored between –15 to –25 °C.手册和实验方案
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